METHOD AND APPARATUS TO PREPARE CARDIAC ORGANOIDS

专利名称：METHOD AND APPARATUS TO PREPARE

CARDIAC ORGANOIDS IN A BIOREACTORSYSTEM

发明人：Kevin David Costa,Timothy James

Cashman,Peter Constantine Backeris

申请号：US15314870申请日：20150529

公开号：US20170107469A1公开日：20170420

摘要：A bioreactor system for preparing a cardiac organoid chamber and forsubsequent testing thereof is described herein and shown in the exemplary drawingfigures. The bioreactor system includes a first vessel having a hollow interior and an opentop. A first cover is mated with the open top of the first vessel. The first cover has a firstopening formed therein. The system further includes a cannula having a lumen thatextends from an open first end to an open second end. The cannula is disposed within thefirst opening of the first cover such that a portion of the cannula lies below the first coverand for insertion into the hollow interior of the first vessel. A porous ring is coupled tothe cannula at or proximate the open second end thereof. The system also includes aballoon catheter having an inflatable balloon at a distal end of a catheter shaft. Theballoon catheter is adapted to pass through the lumen of the cannula when the balloonis in a deflated state. The balloon catheter is axially adjustable within the lumen to allowthe balloon in an inflated state to be disposed adjacent: (1) the open second end of thecannula; and (2) the porous ring for preparing the cardiac organoid chamber about the

inflated balloon and porous ring. The cannula and porous ring construction andcombination allows for the balloon to be deflated and removed from the lumen of thecannula while the engineered cardiac organoid chamber remains attached to the porousring. This permits the testing of organoid pump function, such as organoid pressure andvolume characteristics, without having to transfer the engineered cardiac organoid fromone tool (e.g., an incubation tool) to another tool (e.g., a functional testing apparatus).

申请人：Icahn School of Medicine at Mount Sinai

地址：NEW YORK NY US

国籍：US

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